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Improvement of Opal Multiplex Immunofluorescence Workflow for Human Tissue Sections

2 Apr 2021

Abstract excerpt

The Opal multiplex technique is an established methodology for the detection of multiple biomarkers in one section. The protocol encompasses iterative single stainings and heating-mediated removal of the primary and secondary antibodies after each staining round, leaving untouched the Opal fluorophores which are deposited onto the antigen of interest. According to our experience, repetitive heating of skin...

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PMID: 33797290
DOI: 10.1369/00221554211007793

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