Article
Human papillomavirus genotyping using next generation sequencing (NGS) in cervical lesions: Genotypes by histologic grade and their relative proportion in multiple infections.
PloS one - 1 Jan 2022
Basiletti Jorge Alejandro, Valls Joan, Poklépovich Tomás, Fellner María Dolores, Rol Maryluz, Alonso Rafael, Correa Rita Mariel, Colucci María Celeste, Rodríguez de la Peña Mercedes, Falabella Paula Gabriela, Saíno Agustina, Campos Josefina, Herrero Rolando, Almonte Maribel, Picconi María Alejandra
Abstract excerpt
Sensitive and specific genotyping of human papillomaviruses (HPVs) is critical for the surveillance and monitoring of the vaccine effectiveness. Here, HPV genotypes were identified in 137 cervical samples with different histology (79 ≤CIN1 and 58 CIN3+) using Nested-PCR followed by Next-Generation sequencing (NGS) and relative proportions for each genotype in multiple infections were computed. All samples had...
Topics
- Female
- Humans
- Papillomaviridae
- Alphapapillomavirus
- Papillomavirus Infections
- Genotype
- High-Throughput Nucleotide Sequencing
- Uterine Cervical Neoplasms