Identification of highly specific antibodies for Serine/threonine-protein kinase TBK1 for use in immunoblot, immunoprecipitation and immunofluorescence.

doi:10.12688/f1000research.124632.2

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Article

Identification of highly specific antibodies for Serine/threonine-protein kinase TBK1 for use in immunoblot, immunoprecipitation and immunofluorescence.

F1000Research - 1 Jan 2022

Alshafie Walaa, Fotouhi Maryam, Shlaifer Irina, Ayoubi Riham, Edwards Aled M, Durcan Thomas M, McPherson Peter S, Laflamme Carl

Abstract excerpt

TBK1 is a serine-threonine protein kinase that has been linked to a number of diseases including amyotrophic lateral sclerosis and frontotemporal dementia. Reproducible research on TBK1 has been hampered by the lack of well characterized antibodies. In this study, we characterized 11 commercial antibodies for TBK1 for use in immunoblot, immunofluorescence and immunoprecipitation, using an isogeneic knock-out cell...

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PMID: 36415206
DOI: 10.12688/f1000research.124632.2
PMCID: PMC9647147.2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.