Article
Efficient development of highly polymorphic microsatellite markers based on polymorphic repeats in transcriptome sequences of multiple individuals.
Molecular ecology resources - 1 Jan 2015
Vukosavljev M, Esselink G D, van 't Westende W P C, Cox P, Visser R G F, Arens P, Smulders M J M
Abstract excerpt
The first hurdle in developing microsatellite markers, cloning, has been overcome by next-generation sequencing. The second hurdle is testing to differentiate polymorphic from nonpolymorphic loci. The third hurdle, somewhat hidden, is that only polymorphic markers with a large effective number of alleles are sufficiently informative to be deployed in multiple studies. Both steps are laborious and still performed...
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