Article
Detection of TP53 Mutation in Acute Myeloid Leukemia by RT-PCR-Based Sanger Sequencing.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023
Novak Emily R, Deshpande Anagha, Finlay Darren, Mason James R, Deshpande Aniruddha J, Adams Peter D, Li Sha
Abstract excerpt
The TP53 gene is known to be one of the most frequently mutated genes in various human cancers. In de novo acute myeloid leukemia (AML), TP53 has been found to be mutated in ~10% of patients. Although the frequency of TP53 mutations in AML is substantially lower compared to other human cancers, TP53 mutations in AML are associated with poor response to chemotherapy and poor outcomes. Therefore, assessment of TP53...
Topics
- Humans
- Reverse Transcriptase Polymerase Chain Reaction
- Leukemia, Myeloid, Acute
- Mutation
- Genes, p53
- Base Sequence
- Tumor Suppressor Protein p53