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Article

Dynamic allostery in substrate binding by human thymidylate synthase

1 Sept 2022

Abstract excerpt

Human thymidylate synthase (hTS) is essential for DNA replication and therefore a therapeutic target for cancer. Effective targeting requires knowledge of the mechanism(s) of regulation of this 72 kDa homodimeric enzyme. Here, we investigate the mechanism of binding cooperativity of the nucleotide substrate. We have employed exquisitely sensitive methyl-based CPMG and CEST NMR experiments enabling us to identify...

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PMID: 36200982
DOI: 10.7554/elife.79915

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