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Article

Development of a panel of three multiplex allele-specific qRT-PCR assays for quick differentiation of recombinant variants and Omicron subvariants of SARS-CoV-2.

Frontiers in cellular and infection microbiology - 1 Jan 2022

Li Jianguo, Gao Zefeng, Chen Jing, Cheng Ruiling, Niu Jiahui, Zhang Jialei, Yang You, Yuan Ximei, Xia Juan, Mao Guoli, Liu Hulong, Dong Yongkang, Wu Changxin

Abstract excerpt

Quick differentiation of the circulating variants and the emerging recombinant variants of SARS-CoV-2 is essential to monitor their transmission. However, the widely used gene sequencing method is time-consuming and costly when facing the viral recombinant variants, because partial or whole genome sequencing is required. Allele-specific real time RT-PCR (qRT-PCR) represents a quick and cost-effective method in...

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Identifiers

PMID: 36061868
DOI: 10.3389/fcimb.2022.953027
PMCID: PMC9433905

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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