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Development of multiplex allele-specific RT-qPCR assays for differentiation of SARS-CoV-2 Omicron subvariants.

Applied microbiology and biotechnology - 1 Dec 2024

Li Jianguo, Cheng Ruiling, Bian Zixin, Niu Jiahui, Xia Juan, Mao Guoli, Liu Hulong, Wu Changxin, Hao Chunyan

Abstract excerpt

Quick differentiation of current circulating variants and the emerging recombinant variants of SARS-CoV-2 is essential to monitor their transmissions. However, the widely applied gene sequencing method is time-consuming and costly especially when facing recombinant variants, because a large part or whole genome sequencing is required. Allele-specific reverse transcriptase real time RT-PCR (RT-qPCR) represents a...

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PMID: 38183475
DOI: 10.1007/s00253-023-12941-2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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