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Generation of transgene-free human induced pluripotent stem cells from erythroblasts in feeder-free conditions

17 Aug 2022

Abstract excerpt

This protocol describes the generation and characterization of human induced pluripotent stem cells (hiPSCs) from erythroblasts. A key difference with classical protocols is the reprogramming of erythroblasts from a simple blood draw as opposed to fibroblasts/keratinocytes, which requires a biopsy. Moreover, working with erythroblasts ensures that no recombination of the TCR/BCR genes occurs, as opposed to T...

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PMID: 36035798
DOI: 10.1016/j.xpro.2022.101620

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