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Characterization of a Human Sapovirus Genotype GII.3 Strain Generated by a Reverse Genetics System: VP2 Is a Minor Structural Protein of the Virion.

Viruses - 27 Jul 2022

Li Tian-Cheng, Kataoka Michiyo, Doan Yen Hai, Saito Hiroyuki, Takagi Hirotaka, Muramatsu Masamichi, Oka Tomoichiro

Abstract excerpt

We devised a reverse genetics system to generate an infectious human sapovirus (HuSaV) GII.3 virus. Capped/uncapped full-length RNAs derived from HuSaV GII.3 AK11 strain generated by in vitro transcription were used to transfect HuTu80 human duodenum carcinoma cells; infectious viruses were recovered from the capped RNA-transfected cells and passaged in the cells. Genome-wide analyses indicated no nucleotide...

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Capsid Proteins
Genome-Wide Association Study
Genotype
Reverse Genetics
RNA

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PMID: 36016271
DOI: 10.3390/v14081649
PMCID: PMC9414370

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