Article
Deep sequencing of short capped RNAs reveals novel families of noncoding RNAs.
Genome research - 27 Sept 2022
de Hoon Michiel, Bonetti Alessandro, Plessy Charles, Ando Yoshinari, Hon Chung-Chau, Ishizu Yuri, Itoh Masayoshi, Kato Sachi, Lin Dongyan, Maekawa Sho, Murata Mitsuyoshi, Nishiyori Hiromi, Shin Jay W, Stolte Jens, Suzuki Ana Maria, Tagami Michihira, Takahashi Hazuki, Thongjuea Supat, Forrest Alistair R R, Hayashizaki Yoshihide, Kere Juha, Carninci Piero
Abstract excerpt
In eukaryotes, capped RNAs include long transcripts such as messenger RNAs and long noncoding RNAs, as well as shorter transcripts such as spliceosomal RNAs, small nucleolar RNAs, and enhancer RNAs. Long capped transcripts can be profiled using cap analysis gene expression (CAGE) sequencing and other methods. Here, we describe a sequencing library preparation protocol for short capped RNAs, apply it to a...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.