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Ultra-rapid real-time polymerase chain reaction assay for genotyping of the NT5E gene in Japanese black beef using a mobile PCR device, PCR 1100.

Animal science journal = Nihon chikusan Gakkaiho - 1 Jan 2000

Komatsu Tomohiko, Komatsu Masataka, Nakajima Hiroaki, Uemoto Yoshinobu

Abstract excerpt

The concentration of inosine 5'-monophosphate (IMP) in beef is an important factor contributing to beef palatability. A previous study suggested that single nucleotide polymorphisms (SNPs) in the ecto-5'-nucleotidase (NT5E) gene strongly affect the concentration of IMP under postmortem conditions by regulating NT5E enzymatic activity in beef. Genotyping of the NT5E gene is performed using polymerase chain...

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PMID: 35946783
DOI: 10.1111/asj.13763

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