Article
The clinical application of SNP-based next-generation sequencing (SNP-NGS) for evaluation of chimerism and microchimerism after HLA-mismatched stem cell microtransplantation.
International journal of hematology - 1 Nov 2022
Li Weiyang, Xu Yi, Feng Yufeng, Zhou Haixia, Ma Xiao, Wu Depei, Chen Suning, Sun Aining
Abstract excerpt
Genetic diagnostic methods for evaluation of chimerism after HSCT, such as STR-PCR and XY-FISH, have limited sensitivity. When donor chimerism is in the micro range (< 1%), deviations in the accuracy of assessment are the most significant disadvantage of these common methods. We developed a highly sensitive method that applies SNPs based on NGS in order to explore the value of donor cell microchimerism in...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.