Article
A technical application of quantitative next generation sequencing for chimerism evaluation.
Molecular medicine reports - 1 Oct 2016
Aloisio Michelangelo, Licastro Danilo, Caenazzo Luciana, Torboli Valentina, D'Eustacchio Angela, Severini Giovanni Maria, Athanasakis Emmanouil
Abstract excerpt
At present, the most common genetic diagnostic method for chimerism evaluation following hematopoietic stem cell transplantation is microsatellite analysis by capillary electrophoresis. The main objective was to establish, through repeated analysis over time, if a complete chimerism was present, or if the mixed chimerism was stable, increasing or decreasing over time. Considering the recent introduction of next...
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