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Development of a stable antibody production system utilizing an Hspa5 promoter in CHO cells

24 May 2022

Abstract excerpt

Chinese hamster ovary (CHO) cells are widely used for manufacturing antibody drugs. We attempted to clone a novel high-expression promoter for producing monoclonal antibodies (mAbs) based on transcriptome analysis to enhance the transcriptional abundance of mAb genes. The efficacy of conventional promoters such as CMV and hEF1α decrease in the latter phase of fed-batch cell culture. To overcome this, we screened...

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PMID: 35610229
DOI: 10.1038/s41598-022-11342-1

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