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Article

Impact of SARS-CoV-2 Mutations on PCR Assay Sequence Alignment.

Frontiers in public health - 1 Jan 2022

Negrón Daniel Antonio, Kang June, Mitchell Shane, Holland Mitchell Y, Wist Stephen, Voss Jameson, Brinkac Lauren, Jennings Katharine, Guertin Stephanie, Goodwin Bruce G, Sozhamannan Shanmuga

Abstract excerpt

Real-time reverse transcription polymerase chain reaction (RT-PCR) assays are the most widely used molecular tests for the detection of SARS-CoV-2 and diagnosis of COVID-19 in clinical samples. PCR assays target unique genomic RNA regions to identify SARS-CoV-2 with high sensitivity and specificity. In general, assay development incorporates the whole genome sequences available at design time to be inclusive of...

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Identifiers

PMID: 35570946
DOI: 10.3389/fpubh.2022.889973
PMCID: PMC9096222

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