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Article

Efficient and genotype independent maize transformation using pollen transfected by DNA-coated magnetic nanoparticles.

Journal of integrative plant biology - 1 Jun 2022

Wang Zuo-Ping, Zhang Zhong-Bao, Zheng Deng-Yu, Zhang Tong-Tong, Li Xiang-Long, Zhang Chun, Yu Rong, Wei Jian-Hua, Wu Zhong-Yi

Abstract excerpt

Current gene delivery methods for maize are limited to specific genotypes and depend on time-consuming and labor-intensive tissue culture techniques. Here, we report a new method to transfect maize that is culture-free and genotype independent. To enhance efficiency of DNA entry and maintain high pollen viability of 32%-55%, transfection was performed at cool temperature using pollen pretreated to open the...

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PMID: 35419850
DOI: 10.1111/jipb.13263

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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