Article
Transgenic maize plants by tissue electroporation.
The Plant cell - 1 Dec 1992
D'Halluin K, Bonne E, Bossut M, De Beuckeleer M, Leemans J
Abstract excerpt
In this paper, we describe the transformation of regenerable maize tissues by electroporation. In many maize lines, immature zygotic embryos can give rise to embryogenic callus cultures from which plants can be regenerated. Immature zygotic embryos or embryogenic type I calli were wounded either enzymatically or mechanically and subsequently electroporated with a chimeric gene encoding neomycin phosphotransferase...
Topics
- Base Sequence
- Culture Techniques
- DNA, Recombinant
- DNA, Single-Stranded
- Drug Resistance
- Genetic Markers
- Kanamycin
- Kanamycin Kinase
- Molecular Sequence Data
- Phenotype
- Phosphotransferases