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Generation of human embryonic stem cell-derived lung organoids

4 Apr 2022

Abstract excerpt

This protocol describes how to generate lung organoids from human embryonic stem cells. Lung organoids form by self-assembly in Matrigel and contain lung epithelial cell types. The protocol presented in this study is simple and only uses 6 cytokines or small molecules. This protocol provides a promising tool to study human lung development, drug screening, regeneration, and disease modeling in vitro. For complete...

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PMID: 35403011
DOI: 10.1016/j.xpro.2022.101270

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