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Article

SNP analysis of challenging bone DNA samples using the HID-Ion AmpliSeq™ Identity Panel: facts and artefacts.

International journal of legal medicine - 1 Jul 2023

Fattorini Paolo, Previderè Carlo, Livieri Tommaso, Zupanc Tomaž, Pajnič Irena Zupanič

Abstract excerpt

PCR-MPS is an emerging tool for the analysis of low-quality DNA samples. In this study, we used PCR-MPS to analyse 32 challenging bone DNA samples from three Second World War victims, which previously yielded no results in conventional STR PCR-CE typing. The Identity Panel was used with 27 cycles of PCR. Despite that we only had an average of 6.8 pg of degraded DNA as template, 30 out of 32 libraries (93.8%)...

Topics

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Artifacts
DNA
DNA Fingerprinting
Heterozygote
High-Throughput Nucleotide Sequencing

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Microsatellite Repeats

Polymorphism, Single Nucleotide

Sequence Analysis, DNA

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Identifiers

PMID: 37212920
DOI: 10.1007/s00414-023-03019-9
PMCID: PMC10247551

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026.

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