Article
A novel swab storage gel is superior to dry swab DNA collection, and enables long-range high resolution next generation sequencing HLA typing from buccal cell samples.
HLA - 1 Jun 2022
Shimizu Marie, Takahashi Daisuke, Suzuki Shingo, Shigenari Atsuko, Ito Sayaka, Miyata Shigeki, Satake Masahiro, Matsuhashi Mika, Kulski Jerzy K, Murata Makoto, Azuma Fumihiro, Shiina Takashi
Abstract excerpt
HLA sequence-based DNA typing (SBT) by long-range PCR amplification (LR PCR) and next-generation sequencing (NGS) is a high-throughput DNA sequencing method (LR-NGS-SBT) for the efficient and sensitive detection of novel and null HLA alleles to the field-4 level of allelic resolution without phase ambiguity. However, the accuracy and reliability of the HLA typing results using buccal cells (BCs) and saliva as...
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