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Degenerate sequence-based CRISPR diagnostic for Crimean–Congo hemorrhagic fever virus

10 Mar 2022

Abstract excerpt

CRISPR (clustered regularly interspaced short palindromic repeats), an ancient defense mechanism used by prokaryotes to cleave nucleic acids from invading viruses and plasmids, is currently being harnessed by researchers worldwide to develop new point-of-need diagnostics. In CRISPR diagnostics, a CRISPR RNA (crRNA) containing a "spacer" sequence that specifically complements with the target nucleic acid sequence...

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PMID: 35271569
DOI: 10.1371/journal.pntd.0010285

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