Article
A fast Myosin super enhancer dictates muscle fiber phenotype through competitive interactions with Myosin genes.
Nature communications - 24 Feb 2022
Dos Santos Matthieu, Backer Stéphanie, Auradé Frédéric, Wong Matthew Man-Kin, Wurmser Maud, Pierre Rémi, Langa Francina, Do Cruzeiro Marcio, Schmitt Alain, Concordet Jean-Paul, Sotiropoulos Athanassia, Jeffrey Dilworth F, Noordermeer Daan, Relaix Frédéric, Sakakibara Iori, Maire Pascal
Abstract excerpt
The contractile properties of adult myofibers are shaped by their Myosin heavy chain isoform content. Here, we identify by snATAC-seq a 42 kb super-enhancer at the locus regrouping the fast Myosin genes. By 4C-seq we show that active fast Myosin promoters interact with this super-enhancer by DNA looping, leading to the activation of a single promoter per nucleus. A rainbow mouse transgenic model of the locus...
Topics
- Animals
- Mice
- Mice, Transgenic
- Muscle Fibers, Skeletal
- Muscle, Skeletal
- Myosin Heavy Chains
- Myosins
- Phenotype