Article

Fast, multiplexable and highly efficient somatic gene deletions in adult mouse skeletal muscle fibers using AAV-CRISPR/Cas9

2023-02-20

Abstract excerpt

Molecular screens comparing different disease states to identify candidate genes rely on the availability of fast, reliable and multiplexable systems to interrogate genes of interest. CRISPR/Cas9- based reverse genetics is a promising method to eventually achieve this. However, such methods are sorely lacking for multi-nucleated muscle fibers, since highly efficient nuclei editing is a requisite to robustly inacti...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: c226fdb4-65ea-528f-85af-ddcac409e617
DOI: 10.1101/2023.02.20.529232

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Fast, multiplexable and highly efficient somatic gene deletions in adult mouse skeletal muscle fibers using AAV-CRISPR/Cas9DOI 10.1101/2023.02.20.529232Read

Muscle-specific CRISPR/Cas9 dystrophin gene editing ameliorates pathophysiology in a mouse model for Duchenne muscular dystrophy.Semantic proximity Read

In vivo gene disruption and homology-directed repair in muscles and muscle stem cells using CRISPR/Cas9Semantic proximity Read

Genome Editing of Monogenic Neuromuscular DiseasesSemantic proximity Read

Reversal of molecular pathology by RNA-targeting Cas9 in a myotonic dystrophy mouse modelSemantic proximity Read

Treating neuromuscular diseases: unveiling gene therapy breakthroughs and pioneering future applicationsSemantic proximity Read

CRISPR/Cas correction of muscular dystrophies.Semantic proximity Read

The sustained expression of Cas9 targeting toxic RNAs reverses disease phenotypes in mouse models of myotonic dystrophy type 1.Semantic proximity Read

Muscle-specific gene editing improves molecular and phenotypic defects in a mouse model of myotonic dystrophy type 1.Semantic proximity Read

Precision rewriting of muscle genetics: therapeutic horizons of base and prime editing in skeletal muscle disorders.Semantic proximity Read

Precision and efficacy of RNA-guided DNA integration in high-expressing muscle lociSemantic proximity Read

Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes.Semantic proximity Read

AAV6-mediated systemic shRNA delivery reverses disease in a mouse model of facioscapulohumeral muscular dystrophy.Semantic proximity Read