Article
A new phenotype identification method with the fluorescent expression in cross-sectioned tails in Thy1-GCaMP6s transgenic mice.
Journal of oral science - 1 Apr 2022
Kobayashi Shutaro, O'Hashi Kazunori, Kaneko Keisuke, Kobayashi Satomi, Ogisawa Shouhei, Tonogi Morio, Fujita Satoshi, Kobayashi Masayuki
Abstract excerpt
PURPOSE: Unless the phenotype of the transgenic mice is distinguishable, genotyping in each mouse is required prior to experiments. This study aimed to establish a new identification method for the phenotype in Thy1-GCaMP6s transgenic mice to reduce the cost and time. METHODS: Tail biopsies (2 mm) were performed under general anesthesia with isoflurane in 3 to 4-week-old mice. Then, the resected tail was cut...
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