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Novel split luciferase-based biosensors for evaluation of vitamin D receptor ligands and their application to estimate CYP27B1 activity in living cells.

The Journal of steroid biochemistry and molecular biology - 1 Oct 2018

Mano Hiroki, Ikushiro Shinichi, Sakaki Toshiyuki

Abstract excerpt

Recently, we successfully generated a novel detection system for vitamin D receptor (VDR) ligands in vivo and in vitro, using a split-luciferase technique called the LucN-LBD-LucC biosensor that is a chimeric fusion protein of firefly luciferase with the ligand binding domain (LBD) of VDR. In this system, the luciferase light intensity of the LucN-LBD-LucC biosensor was decreased by binding of VDR ligands....

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25-Hydroxyvitamin D3 1-alpha-Hydroxylase
Biosensing Techniques
Chlorocebus aethiops
COS Cells
Ligands

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Receptors, Calcitriol

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PMID: 30004013
DOI: 10.1016/j.jsbmb.2018.06.017

Genes

VDR
SRC

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