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Detection of Tri-allelic Single Nucleotide Polymorphisms of ABCB1 and CRP Genes by Penta-Primer Amplification Refractory Mutation System-Polymerase Chain Reaction.

Genetic testing and molecular biomarkers - 1 Jan 2022

Yang Siqi, Shi Huiting, Zhang Zhanfa, Chen Zhuochen, Yang Weiming, Zhu Weifeng

Abstract excerpt

Objective: The standard methods for tri-allelic single nucleotide polymorphism (SNP) genotyping require special equipment and are costly to perform. The aim of this study was to establish a fast, simple, and low-cost method to differentiate among tri-allelic SNPs in general laboratories. Methods: Based on the tetra-primer amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR) typing of...

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PMID: 35089072
DOI: 10.1089/gtmb.2021.0145

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