Article
Phenotypic Characterization of SLex+ and CLA+ CD4+ T Cells.
STAR protocols - 18 Dec 2020
Kuri-Cervantes Leticia, Pampena Maria Betina, Betts Michael R
Abstract excerpt
Recent advances in high-resolution multiparametric flow cytometry enable ever deeper analysis of human lymphocyte subsets that require rigorous methodology development and optimization. Here, we detail methods to characterize glycosylated Sialyl-LewisX (SLeX)- or cutaneous lymphocyte-associated antigen (CLA)-expressing CD4+ T cells using two separate multiparametric flow cytometry panels enabling the...
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