Article
Nanopore-Based Direct RNA-Sequencing Reveals a High-Resolution Transcriptional Landscape of Porcine Reproductive and Respiratory Syndrome Virus.
Viruses - 16 Dec 2021
Zhang Riteng, Wang Peixin, Ma Xin, Wu Yifan, Luo Chen, Qiu Li, Zeshan Basit, Yang Zengqi, Zhou Yefei, Wang Xinglong
Abstract excerpt
The TRS-mediated discontinuous transcription process is a hallmark of Arteriviruses. Precise assessment of the intricate subgenomic RNA (sg mRNA) populations is required to understand the kinetics of viral transcription. It is difficult to reconstruct and comprehensively quantify splicing events using short-read sequencing, making the identification of transcription-regulatory sequences (TRS) particularly...
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