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Rapid Detection of Clostridium tetani by Recombinase Polymerase Amplification Using an Exo Probe

24 Nov 2021

Abstract excerpt

detected in less than 20 min, with a detection limit of 20 copies/reaction. The RPA assay displayed good selectivity, and there were no cross-reactions with other infectious bacteria common in penetrating wounds. Tests of target-spiked serum and pus extract revealed that RPA is robust to interfering factors and has great potential for further development for biological sample analysis. This method has been...

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PMID: 34818665
DOI: 10.4014/jmb.2109.09022

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