Inclusion of a degron reduces levels of undesired inteins after AAV-mediated protein trans-splicing in the retina

Abstract excerpt

Split intein-mediated protein trans- splicing expands AAV transfer capacity, thus overcoming the limited AAV cargo. However, non-mammalian inteins persist as trans- splicing by-products, and this could raise safety concerns for AAV intein clinical applications. In this study, we tested the ability of several degrons to selectively decrease levels of inteins after protein trans- splicing and found that a version...

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