Article
A systematic genome-wide mapping of oncogenic mutation selection during CRISPR-Cas9 genome editing.
Nature communications - 11 Nov 2021
Sinha Sanju, Barbosa Karina, Cheng Kuoyuan, Leiserson Mark D M, Jain Prashant, Deshpande Anagha, Wilson David M, Ryan Bríd M, Luo Ji, Ronai Ze'ev A, Lee Joo Sang, Deshpande Aniruddha J, Ruppin Eytan
Abstract excerpt
Recent studies have reported that genome editing by CRISPR-Cas9 induces a DNA damage response mediated by p53 in primary cells hampering their growth. This could lead to a selection of cells with pre-existing p53 mutations. In this study, employing an integrated computational and experimental framework, we systematically investigated the possibility of selection of additional cancer driver mutations during...
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