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Article

Protocol for assessment of the efficiency of CRISPR/Cas RNP delivery to different types of target cells

9 Nov 2021

Abstract excerpt

BACKGROUND: Delivery of CRISPR/Cas RNPs to target cells still remains the biggest bottleneck to genome editing. Many efforts are made to develop efficient CRISPR/Cas RNP delivery methods that will not affect viability of target cell dramatically. Popular current methods and protocols of CRISPR/Cas RNP delivery include lipofection and electroporation, transduction by osmocytosis and reversible permeabilization and...

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PMID: 34752487
DOI: 10.1371/journal.pone.0259812

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