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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytes

10 Sept 2021

Abstract excerpt

Adeno-associated virus serotype 6 (AAV6) is a valuable reagent for genome editing of hematopoietic cells due to its ability to serve as a homology donor template. However, a comprehensive study of AAV6 transduction of hematopoietic cells in culture, with the goal of maximizing ex vivo genome editing, has not been reported. Here, we evaluated how the presence of serum, culture volume, transduction time, and...

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PMID: 34703842
DOI: 10.1016/j.omtm.2021.09.003

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