Article
Single nucleotide polymorphisms and copy-number variations in the Trypanosoma brucei repeat (TBR) sequence can be used to enhance amplification and genotyping of Trypanozoon strains.
PloS one - 1 Jan 2021
Van Reet Nick, Patient Pyana Pati, Dehou Sara, Bebronne Nicolas, Deborggraeve Stijn, Büscher Philippe
Abstract excerpt
The Trypanosoma brucei repeat (TBR) is a tandem repeat sequence present on the Trypanozoon minichromosomes. Here, we report that the TBR sequence is not as homogenous as previously believed. BLAST analysis of the available T. brucei genomes reveals various TBR sequences of 177 bp and 176 bp in length, which can be sorted into two TBR groups based on a few key single nucleotide polymorphisms. Conventional and...
Topics
- DNA Copy Number Variations
- DNA, Protozoan
- Polymorphism, Single Nucleotide
- Protozoan Proteins
- Repetitive Sequences, Nucleic Acid
- Trypanosoma brucei brucei
- Trypanosomiasis, African