Article
Reconstitution of an intact clock reveals mechanisms of circadian timekeeping.
Science (New York, N.Y.) - 8 Oct 2021
Chavan Archana G, Swan Jeffrey A, Heisler Joel, Sancar Cigdem, Ernst Dustin C, Fang Mingxu, Palacios Joseph G, Spangler Rebecca K, Bagshaw Clive R, Tripathi Sarvind, Crosby Priya, Golden Susan S, Partch Carrie L, LiWang Andy
Abstract excerpt
Circadian clocks control gene expression to provide an internal representation of local time. We report reconstitution of a complete cyanobacterial circadian clock in vitro, including the central oscillator, signal transduction pathways, downstream transcription factor, and promoter DNA. The entire system oscillates autonomously and remains phase coherent for many days with a fluorescence-based readout that...
Topics
- Bacterial Proteins
- Circadian Rhythm
- Circadian Rhythm Signaling Peptides and Proteins
- Gene Expression Regulation, Bacterial
- Molecular Mimicry
- Mutation
- Phosphotransferases
- Promoter Regions, Genetic
- Protein Domains