Article
Characterization of recombinant pumpkin 2S albumin and mutation studies to unravel potential DNA/RNA binding site.
Biochemical and biophysical research communications - 26 Nov 2021
Savita Brajesh Kumar, Dalal Vikram, Choudhary Shweta, Gupta Deena Nath, Das Neeladrisingha, Tomar Shailly, Kumar Pravindra, Roy Partha, Sharma Ashwani Kumar
Abstract excerpt
The native pumpkin 2S albumin, a multifunctional protein, possess a variety of potential biotechnologically exploitable properties. The present study reports the characterization of recombinant pumpkin 2S albumin (rP2SA) and unraveling of its potential DNA/RNA binding site. The purification and characterization of the rP2SA established that it retains the characteristic α-helical structure and exhibited...
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