Article
The role of calcium binding to the EF-hand-like motif in bacterial solute-binding protein for alginate import.
Bioscience, biotechnology, and biochemistry - 24 Nov 2021
Okumura Kenji, Maruyama Yukie, Takase Ryuichi, Mikami Bunzo, Murata Kousaku, Hashimoto Wataru
Abstract excerpt
Gram-negative Sphingomonas sp. A1 incorporates acidic polysaccharide alginate into the cytoplasm via a cell-surface alginate-binding protein (AlgQ2)-dependent ATP-binding cassette transporter (AlgM1M2SS). We investigated the function of calcium bound to the EF-hand-like motif in AlgQ2 by introducing mutations at the calcium-binding site. The X-ray crystallography of the AlgQ2 mutant (D179A/E180A) demonstrated the...
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