Article
Alginate Polymerization and Modification Are Linked in Pseudomonas aeruginosa.
mBio - 12 May 2015
Moradali M Fata, Donati Ivan, Sims Ian M, Ghods Shirin, Rehm Bernd H A
Abstract excerpt
UNLABELLED: The molecular mechanisms of alginate polymerization/modification/secretion by a proposed envelope-spanning multiprotein complex are unknown. Here, bacterial two-hybrid assays and pulldown experiments showed that the catalytic subunit Alg8 directly interacts with the proposed copolymerase Alg44 while embedded in the cytoplasmic membrane. Alg44 additionally interacts with the lipoprotein AlgK bridging...
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