Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

CRISPR-Cas9 Targeted Mutagenesis Leads to Simultaneous Modification of Different Homoeologous Gene Copies in Polyploid Oilseed Rape (Brassica napus).

Plant physiology - 1 Jun 2017

Braatz Janina, Harloff Hans-Joachim, Mascher Martin, Stein Nils, Himmelbach Axel, Jung Christian

Abstract excerpt

In polyploid species, altering a trait by random mutagenesis is highly inefficient due to gene redundancy. We have stably transformed tetraploid oilseed rape (Brassica napus) with a CRISPR-Cas9 construct targeting two ALCATRAZ (ALC) homoeologs. ALC is involved in valve margin development and, thus, contributes to seed shattering from mature fruits. Knocking out ALC would increase shatter resistance to avoid seed...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Brassica napus
Share to CRISPR-Cas Systems

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gene Dosage

Share to Gene Targeting

Share to Inheritance Patterns

Share to Mutagenesis, Insertional

Share to Polyploidy

Share to Transformation, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 28584067
DOI: 10.1104/pp.17.00426
PMCID: PMC5462057

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

CRISPR-Cas9 Targeted Mutagenesis Leads to Simultaneous Modification of Different Homoeologous Gene Copies in Polyploid Oilseed Rape (Brassica napus).PMID 28584067Read ↗

CRISPR/Cas9-mediated genome editing efficiently creates specific mutations at multiple loci using one sgRNA in Brassica napus.Semantic proximity Read ↗

GLABRA2-based selection efficiently enriches Cas9-generated nonchimeric mutants in the T1 generation.Semantic proximity Read ↗

Gene Editing in Polyploid Crops: Wheat, Camelina, Canola, Potato, Cotton, Peanut, Sugar Cane, and Citrus.Semantic proximity Read ↗

CRISPRing into the woods.Semantic proximity Read ↗

CRISPR/Cas9-Mediated Multiplex Genome Editing of JAGGED Gene in Brassica napus L.Semantic proximity Read ↗

Generation and Inheritance of Targeted Mutations in Potato (Solanum tuberosum L.) Using the CRISPR/Cas System.Semantic proximity Read ↗

Spontaneous, Artificial, and Genome Editing-Mediated Mutations in Prunus.Semantic proximity Read ↗

Characterization of SHATTERPROOF Homoeologs and CRISPR-Cas9-Mediated Genome Editing Enhances Pod-Shattering Resistance in Brassica napus L.Semantic proximity Read ↗

CRISPR-Cas9-mediated chromosome engineering in Arabidopsis thaliana.Semantic proximity Read ↗

Site-directed mutagenesis in Arabidopsis thaliana using dividing tissue-targeted RGEN of the CRISPR/Cas system to generate heritable null alleles.Semantic proximity Read ↗

Engineering homoeologs provide a fine scale for quantitative traits in polyploid.Semantic proximity Read ↗

Continual improvement of multiplex mutagenesis in ArabidopsisSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.