Article
A bioluminescent and homogeneous SARS-CoV-2 spike RBD and hACE2 interaction assay for antiviral screening and monitoring patient neutralizing antibody levels.
Scientific reports - 16 Sept 2021
Alves Juliano, Engel Laurie, de Vasconcelos Cabral Renata, Rodrigues Eduardo L, de Jesus Ribeiro Liane, Higa Luiza M, da Costa Ferreira Júnior Orlando, Castiñeiras Terezinha Marta P P, de Carvalho Leitão Isabela, Tanuri Amilcar, Goueli Said A, Zegzouti Hicham
Abstract excerpt
Here we describe a homogeneous bioluminescent immunoassay based on the interaction between Fc-tagged SARS-CoV-2 Spike RBD and human ACE2, and its detection by secondary antibodies labeled with NanoLuc luciferase fragments LgBit and SmBit. The assay utility for the discovery of novel inhibitors was demonstrated with a panel of anti-RBD antibodies, ACE2-derived miniproteins and soluble ACE2. Studying the effect of...
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