Article
Activation of CheY mutant D57N by phosphorylation at an alternative site, Ser-56.
Molecular microbiology - 1 Dec 1999
Appleby J L, Bourret R B
Abstract excerpt
The site of phosphorylation of the chemotaxis response regulator CheY is aspartate 57. When Asp-57 is replaced with an asparagine, the resultant protein can be phosphorylated at an alternative site. We report here that phosphorylation of this mutant protein, CheY D57N, at the alternative site affords the protein activity in vivo in the absence of CheZ. Using a direct phosphopeptide mapping approach, we identified...
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