Article
Conformation sensitive gel electrophoresis for the detection of calreticulin mutations in BCR-ABL1-negative myeloproliferative neoplasms.
International journal of laboratory hematology - 1 Dec 2021
Zakaria Nur Atikah, Rosle Norfifiana Alisa, Siti Asmaa Mat Jusoh, Aziee Sudin, Haiyuni Mohd Yassim, Samat Nurul Ameera, Husin Azlan, Hassan Rosline, Ramli Marini, Mohamed Yusoff Shafini, Ibrahim Ibrahim Khidir, Al-Jamal Hamid Ali Nagi, Johan Muhammad Farid
Abstract excerpt
INTRODUCTION: Calreticulin (CALR) mutations in myeloproliferative neoplasms (MPN) have been reported to be key markers in the molecular diagnosis, particularly in patients lacking JAK2 V617F mutation. In most current reports, CALR mutations were analysed by either allele-specific PCR (AS-PCR), or the more expensive quantitative real-time PCR, pyrosequencing and next-generation sequencing. Hence, we report the use...
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