Article
PCR-RFLP molecular confirmation of color dilution alopecia in dogs in Brazil.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc - 1 Sept 2021
Caramalac Silvana M, Caramalac Simone M, Babo-Terra Veronica J, Ramos Carlos A N, Palumbo Mariana I P
Abstract excerpt
Color dilution alopecia (CDA) is a dermatopathy observed exclusively in animals having a diluted coat color. In dogs, color dilution occurs as a result of a single-nucleotide variation (SNV) c.-22G > A in the melanophilin gene. We standardized a PCR-restriction-fragment length polymorphism (PCR-RFLP) technique to identify this mutation and determine its frequency in dogs in Brazil. The standardized PCR-RFLP...
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