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Anion solvation enhanced by positive supercharging mutations preserves thermal stability of an antibody in a wide pH range.

Biochemical and biophysical research communications - 23 Jul 2021

Kasahara Keisuke, Kuroda Daisuke, Tanabe Aki, Kawade Raiji, Nagatoishi Satoru, Tsumoto Kouhei

Abstract excerpt

Proteins function through interactions with other molecules. In protein engineering, scientists often engineer proteins by mutating their amino acid sequences on the protein surface to improve various physicochemical properties. "Supercharging" is a method to design proteins by mutating surface residues with charged amino acids. Previous studies demonstrated that supercharging mutations conferred better thermal...

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Identifiers

PMID: 34058475
DOI: 10.1016/j.bbrc.2021.05.053

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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