Article
The effect of charge mutations on the stability and aggregation of a human single chain Fv fragment.
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V - 1 Jun 2017
Austerberry James I, Dajani Rana, Panova Stanislava, Roberts Dorota, Golovanov Alexander P, Pluen Alain, van der Walle Christopher F, Uddin Shahid, Warwicker Jim, Derrick Jeremy P, Curtis Robin
Abstract excerpt
The aggregation propensities for a series of single-chain variable fragment (scFv) mutant proteins containing supercharged sequences, salt bridges and lysine/arginine-enriched motifs were characterised as a function of pH and ionic strength to isolate the electrostatic contributions. Recent improvements in aggregation predictors rely on using knowledge of native-state protein-protein interactions. Consistent with...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.