Article
Sheath fluid impacts the depletion of cellular metabolites in cells afflicted by sorting induced cellular stress (SICS).
Cytometry. Part A : the journal of the International Society for Analytical Cytology - 1 Sept 2021
Ryan Kamilah, Rose Rebecca E, Jones Drew R, Lopez Peter A
Abstract excerpt
Flow cytometrists have long observed a spectrum of cell-type-specific changes ranging from minor functional defects to outright cell destruction after purification of cells using conventional droplet cell sorters. We have described this spectrum of cell perturbations as sorter induced cellular stress, or SICS (Lopez and Hulspas, Cytometry, 2020, 97, 105-106). Despite the potential impact of this issue and...
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