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Article

Comparison of active site mutations at subsite + 2 of Anoxybacillus ayderensis A9 β-glucosidase for hydrolysis of pNPG and polydatin.

BMC biotechnology - 1 Jul 2025

Zada Numan Saleh, Belduz Ali Osman, Alessa Abdulrahman H, Güler Halil Ibrahim, Karaoğlan Mine, Badshah Malik, Shah Aamer Ali, Kalsoom Aasia, Khan Samiullah

Abstract excerpt

β-glucosidase from Anoxybacillus ayderensis A9 (BglA9) is a potent enzyme for enzymatic hydrolysis of polydatin to resveratrol. Based on structural and bioinformatics analysis an area near + 2 subsite of the active site pocket of BglA9 was selected and single point mutations were introduced with the aim to enhance the catalytic efficiency of the enzyme towards pNPG and polydatin. The active site region selected...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 40598098
DOI: 10.1186/s12896-025-00984-4
PMCID: PMC12211328

Genes

GH1

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