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TILLING-by-Sequencing+ to Decipher Oil Biosynthesis Pathway in Soybeans: A New and Effective Platform for High-Throughput Gene Functional Analysis.

International journal of molecular sciences - 19 Apr 2021

Lakhssassi Naoufal, Zhou Zhou, Cullen Mallory A, Badad Oussama, El Baze Abdelhalim, Chetto Oumaima, Embaby Mohamed G, Knizia Dounya, Liu Shiming, Neves Leandro G, Meksem Khalid

Abstract excerpt

Reverse genetic approaches have been widely applied to study gene function in crop species; however, these techniques, including gel-based TILLING, present low efficiency to characterize genes in soybeans due to genome complexity, gene duplication, and the presence of multiple gene family members that share high homology in their DNA sequence. Chemical mutagenesis emerges as a genetically modified-free strategy...

Topics

Fatty Acid Desaturases
Mutation
Plant Proteins
Soybean Oil
Glycine max

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 33921707
DOI: 10.3390/ijms22084219
PMCID: PMC8073088

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