Article
Construction of a high-density mutant library in soybean and development of a mutant retrieval method using amplicon sequencing.
BMC genomics - 26 Nov 2015
Tsuda Mai, Kaga Akito, Anai Toyoaki, Shimizu Takehiko, Sayama Takashi, Takagi Kyoko, Machita Kayo, Watanabe Satoshi, Nishimura Minoru, Yamada Naohiro, Mori Satomi, Sasaki Harumi, Kanamori Hiroyuki, Katayose Yuichi, Ishimoto Masao
Abstract excerpt
BACKGROUND: Functions of most genes predicted in the soybean genome have not been clarified. A mutant library with a high mutation density would be helpful for functional studies and for identification of novel alleles useful for breeding. Development of cost-effective and high-throughput protocols using next generation sequencing (NGS) technologies is expected to simplify the retrieval of mutants with mutations...
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